Wednesday, August 23, 2017

KAATSU Training On Proliferation and Differentiation Of Goat Bone Marrow Mesenchymal Stem Cells



The video above shows the subjects of an interesting series of research projects by the China-Japan Union Hospital of Jilin University in Changchun, China.

The initial results were published in the Chinese language in the Chinese Journal of Laboratory Diagnosis (25 Aug 2016 issue, Vol. 20, No. 8, P. 1240) entitled Effects of KAATSU Training on proliferation and differentiation of goat bone marrow mesenchymal stem cells by by Yu-hui Yang, Shao-qian Sun, Yoshiaki Sato, et al.

The English translation of the paper is below:

Objective

To explore the effects of KAATSU Training on proliferation and differentiation of goat bone marrow mesenchymal stem cells.

Methods

60 Boer goats were randomly divided into experimental group and control group, the experimental group was given KAATSU Training twice a week, non-KAATSU Training twice a week for the control group. 6 months later, we got the goat bone marrow and then separated and absorbed the white cloud layer which mainly contained the mononuclear cell in the upper-middle interface with the method of percoll-density gradient centrifugation, cultured and observed the cell morphology and the proliferation rate; the cells of the two groups were induced into cardiomyocyte like cells by the 5-azacytidine. The cells which had been differentiated were detected with the expression of the cardiac specific antigen α-actin by immunofluorescence assay.

Results

The cells isolated from the bone marrow in the white cloud layer were adherent, generated and grew well. In addition, the cells which induced by 5-azacytidine could express the cardiac specific antigen. The bone marrow mesenchymal stem cells of the experimental group were small and round, and their proliferation rate was faster than the control group, though the shape of the cells in the control group were polygonal or triangular, and the proliferation rate were slow.

Conclusion

It has been succeeded both in separation and cultivation of the bone marrow mesenchymal stem cells, and also induced the proliferation of turning into cardiomyocyte like cells in vitro. The bone marrow mesenchymal stem cells in pressurization motion for a long period of time were easier to proliferate than the cells in non-pressurization motion, but the differentiated capability were low.

Bone marrow mesenchymal stem cells (BMSCs) are from the mesoderm and are pluripotent stem cells with high capability in proliferation, self-renewal and multi-directional differentiation potential. Further studies have demonstrated that BMSCs can differentiate into cardiomyocytes, neurons or neuroglial cells. Upon in vivo transplantation, these cells can migrate to injured sites (mostly to ischemic or anaerobic sites) and repair respective tissues. Cell transplantation has provided brand-new treatment strategy to irreversible heart diseases. BMSCs are currently considered as one of the most ideal seed cells for cell transplantation, and are often used as carrier cells in gene therapy. Allogeneic BMSC transplantation may trigger immunologic rejection, while autologous stem cells are of limited quantities.

It is therefore crucial to look into how autologous stem cells could proliferate and be release to the bloodstream, especially in large mammals. In recent years, the number of studies focused on small animals such as mice/rat or rabbit is relatively high, but few studies and report investigate into BMSCs in bigger animals like goats. Thus, it is important to study the in vitro directed differentiation of BMSCs from goat as a big animal.


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